Okazaki proposed the replication is not continuous on both

PCR is another in vitro DNA synthesis reaction
The twist in this case is that the reaction is repeated 25-30 times so that the DNA between
the primers is amplified
The first PCR cycle:
The sequence between the two primers will be
amplified
Four cycles of PCR
Copy number of the sequence
between the primers increases
exponentially
SEQUENCIAMENTO DE DNA
Profa. Dra. Maria Aparecida Fernandez
Depto de Biologia Celular e Genética
Universidade Estadual de Maringá
Structure of dideoxynucleotide triphosphates
Dideoxy DNA sequencing is an in vitro DNA
synthesis reaction with a twist
DNAP requires a
template and a primer
The primer is labeled so the DNA
fragments synthesized can be
detected by autoradiography.
The [ddNTP]
determines the
distribution of
chain lengths
produced.
Alto peso molecular
Filme de raio X
Auto-radiograma
Leitura manual
Baixo peso molecular
TGGCTCGGCCTCAAGTCGAG
GTTATCAGATCTGCAACTCAA
Automated DNA Sequencing
Reação de seqüênciamento
Fragmentos amplificados na reação de seqüênciamento
Typical output of an automated sequencer
Eletroforese no seqüênciamento
Captura do fluorescente e processamento da informação
Seqüenciador automático
Conjunto
de
16
capilares
Panorama no momento da corrida
Análise preliminar pós corrida
ELETROFEROGRAMAS