CRB-SAÚDE

Biological Resource Center
for Health
Fundação Oswaldo Cruz
CRB-SAÚDE
The start of BRC at Fiocruz: CLIOC
May 2005 – Workshop
CRBs and Conformity
Assessment for Biological
Material
In loco audits in the
collections candidates
to the accreditation (ISO
17025)
Establishement of the
prototype of Brazilian
CRB Network
4 collections were selected:
CLIOC - FIOCRUZ
CBMAI - UNICAMP
CENARGEN - EMBRAPA
Banco de Células - UFRJ
Selection of
collections able to
accredited by
INMETRO
July 2005 - Internal
Auditing Training on
ISO/17025:2001
CLIOC: Model for the BRC at Fiocruz
Activities developed within the BRC project
•
Conclusion of the physical infra-structure (space remodelling)
•
Establishment of the Quality Management System (NBR ISO 17025)
•
Adequacy to the OECD guidelines
•
Inventory process
•
Definition of the ideal method for preservation and molecular techniques
developed to determine the levels of intra-specific biodiversity
Research
•
•
•
•
•
•
Microsatelites
Multi-locus sequencing typing
Comparative genome
Taxonomic markers
Drug resistance markers
Functional studies: growth curve, gene expression
CLIOC: Model for the BRC at Fiocruz
Year of 2009 (Strains)
CHARACTERIZED
DEPOSITED
DONNATED
90
156
123
TOTAL = 369
Services offered by CLIOC to
• research
• development of vaccine and diagnostic kits projects
• production of diagnostic kits
Bacteria Collections
I. Coleções de Bactérias
1.1. Coleções de Bactérias do IOC:
- Coleção de Culturas de Bactérias de Interesse em Saúde:
- Enteropatógenos Bacterianos
- Bactérias de Origem Hospitalar
- Gênero Listeria
- Gênero Campylobacter
- Coleção de Culturas do Gênero Bacillus e Gêneros Correlatos
- Coleção de Bactérias da Mata Atlântica
1.2. Coleções de Bactérias do IAM:
- Coleção de Culturas de Yersinia
- Coleção de Bactérias NB2 do IAM
1.3. Coleção de Microorganismos de Referência do INCQS – Domínio
Bactérias
1.4. Coleção de Bactérias da Amazônia do ILMD
Fungi Collections
II. Coleções de Fungos
2.1. Coleções de Fungos do IOC:
- Coleção de Culturas de Fungos do IOC – CCF/IOC
- Coleção Micológica de Trichocomaceae
2.2. Coleção de Microorganismos de Referência do INCQS – Domínio
Fungos
2.3. Coleção de Fungos Patogênicos do IPEC
2.4. Coleção de Fungos da Amazônia do ILMD
Protozoa and Virus Collections
III. Coleções de Protozoários
3.1. Coleções de Protozoários do IOC:
- Coleção de Trypanosoma de Reservatórios
Silvestres, Domésticos e Vetores
- Coleção de Leishmania do IOC – CLIOC/IOC
3.2. Coleções de Protozoários do IGM
IV. Coleções de Vírus
4.1. Banco de Vírus do LAVITE do IAM
14 microbial collections
Microbial Collections – FINEP/Modernit (2007)
– Coleção de Culturas do Gênero Bacillus e Gêneros Correlatos
do IOC
– Coleção de Culturas de Bactérias de Interesse em Saúde do
IOC
– Coleção de Culturas de Fungos do IOC
– Coleção Micológica de Trichocomaceae do IOC
– Coleção de Leishmania do IOC
– Coleção de Bactérias da Amazônia do ILMD
– Coleção de Fungos da Amazônia do ILMD
Quality Environmental Project: “Cleaner Research” P+L
water
consumption
Reverse osmosis
Partnership with
↓ energy
consumption
• Calibration of
equipments
• Equipment
substitution
• Sensitization
process to optimize
the use of
equipments
• Paper recycling
↓ residues
consumption
Chemical
management, storage
and discard
Microbial Collections – FINEP/CRB-FIOCRUZ (2008)
– Coleção de Culturas do Gênero Bacillus e Gêneros Correlatos do
IOC
– Coleção de Culturas de Bactérias de Interesse em Saúde do IOC
– Coleção de Culturas de Fungos do IOC
– Coleção Micológica de Trichocomaceae do IOC
– Coleção de Bactérias da Amazônia do ILMD
– Coleção de Fungos da Amazônia do ILMD
– Coleção de Bactérias da Mata Atlântica do IOC
– Coleção de Trypanosoma de Reservatórios Silvestres, Domésticos
e Vetores do IOC
– Coleção de Fungos Patogênicos do IPEC
– Coleção de Microrganismos de Referência do INCQS
Fiocruz Biological
Collections
Collection webpage s
visibility
on-line access to the
service: strain
identification, deposit
and distribution
Strain catalogue
http://clioc.ioc.fiocruz.br
Science & technology in health
– Biological and biomedical research: basic laboratory
research; traditional disciplines (such as parasitology,
microbiology, immunology, genetics, etc.) and new
disciplines (such as genomics and pos-genomics), reorganized in complex scenario
– Public health research: health systems and services;
epidemiology and surveillance
– Clinical research: studies on individual and group
population; clinical assays
– Technological research: innovation in medicines,
vaccines and diagnostic
Problems in C&T in health (1)
– National fragility on health innovation
– High dependency of knowledge generated on other
countries
– Industry vulnerability of the health industrial complex
– Disarticulation among industrial, technological and
health policies
Problems in C&T in health
– Dissociation between research and burden of diseases
(particularly concerning neglected diseases)
– Individual health prevails over collective health;
– Knowledge produced is scarcely utilized for policies and health
program definition
– Deficient structure for biotechnology
– Weakness of the system for scientific production assessment
Fiocruz must contribute to overcome this technological
and economical gap with R&D and production
Worldmapper: area of countries proportional to
physicians working
From C Morel, 2009
Worldmapper: area of countries proportional to
HIV/AIDS prevalence
From C Morel, 2009
Worldmapper: area of countries proportional to
malaria cases
From C Morel, 2009
The Sisyphus challenge in Chile
• “Developing countries share disbelief
about the benefits of the endogenous
production of science as a tool for
economical growth. Hence, public policies
to strengthen science and technology and
promote the culture of innovation are, in
general, weak and sometimes incoherent”
Scientometrics 72(1):93-103, 2007
From C Morel, 2009
On-going initiatives in the country
National Conferences on Science, Technology and
Health Innovation (2000, 2004, 2010)
National Agenda of Priorities on Health Research
Private-Public Partnership (PPP) and Innovation Laws
Innovation policy: pharmaceutic (BNDES)
Creation of ABDI and INPI re-organization
Competitiveness forum of Biotechnology
Expansion of offer and quality of pos-graduation
programs on health
Fiocruz Response
Production of:
– Vaccines of the National Immunization
Program, in collaboration with Instituto
Butantan (SP)
– Diagnostic kits for the main infectious disease
– Anti-retrovirals and drugs for controlling and
treating the main endemies (malaria, TB,
schistosomiasis, leishmaniasis, etc)
– Bio-drugs: α−interferon, erythropoietin
– Medicines of continual use (hypertension,
diabetes)
Health-BRC
CRB-Saúde (Health-BRC)
VISION
To build a collection – unique in the world – of biological
material related mainly to neglected diseases of Latin
America
in
order
to
support
scientific
research,
epidemiological studies, as well as the development and
production of bioproducts directed to diagnosis vaccine and
drugs.
CRB-Saúde (Health-BRC)
MISSION
To
collect,
preserve,
indentify
and
catalogue,
following
international standards of Quality, a representative collection of
microrganisms which are relevant to epidemiology surveillance,
particularly those related to neglected diseases, and to the
technological development of products
human Health.
with significance to
CRB-Saúde (Health-BRC)
(Primary) scope
Virus
Bacterial cultures
Fungi cultures
Protozoa cultures
CRB-Saúde (Health-BRC)
Staff
Specialists in the following fields: microbiology, parasitology,
genetics, cellular biology, molecular biology, and/or tropical
medicine
Topics of interest
Taxonomy
Evolution
Molecular phylogeny
Microbial diversity
Molecular epidemiology
Development and evaluation of cultivation and preservation methods
Working Plan for the BRC Network: Health-BRC
Meta
Etapa
1. Elaboração do Projeto CRB-Saúde
1.1. Definição de parcerias nacionais e estrangeiras
1.2. Participação em reuniões estratégicas
1.3. Chamada institucional para definir a composição do
acervo
1.4. Visita e avaliação das coleções proponentes
1.5. Estruturação do projeto
2. Construção do prédio do CRBSaúde
2.1. Licitação e contratação de empresa para elaboração dos
projetos básico e executivo
2.2. Elaboração do projeto básico
2.3. Elaboração do projeto executivo
2.4. Licitação para a construção do prédio
2.5. Construção do Prédio
3. Aparelhamento do CRB-Saúde
3.1. Elaboração do Termo de Referência para licitação para
aquisição dos equipamentos
3.2. Licitações para aquisição dos equipamentos
3.3. Aquisição e recebimento dos equipamentos
3.4. Instalação dos equipamentos
Proposal of a model for biological material management
POST
POSTANALYTICAL
ANALYTICAL
PREANALYTICAL
Health Centers , Outpatients units,
Hospitals, universities
BIOLOGICAL
MATERIAL
RECEPTION
CENTER
Lab
Lab
Lab
Lab
RESEARCH & TDI
Lab
FINAL RESULT
QUALITY CONTROL
Lab
COLLECTIONS
BRC
SVS
Epidemiological
surveillance