GH53 Endo-beta-1,4-Galactanase from a newly isolated Bacillus

GH53 Endo-beta-1,4-Galactanase from a newly isolated Bacillus licheniformis
CBMAI 1609 as an enzymatic cocktail supplement for biomass
saccharification
Evandro Antônio de Lima
1,3
, Carla Botelho Machado 1, Letícia Maria Zanphorlin 1,
Richard John Ward 1,2, Helia Harumi Sato 3 and Roberto Ruller 1*
(1) Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional
de Pesquisa em Energia e Materiais (CNPEM), Campinas, Brasil
(2) Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão
Preto, Universidade de São Paulo, Brasil
(3) Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, Brasil
* Corresponding author
Email address: [email protected]
Address: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro
Nacional de Pesquisa em Energia e Materiais (CNPEM), Caixa Postal 6192, CEP 13083970, Campinas, São Paulo, Brasil.
Fax: +55 19 3512 1004.
Phone.: +55 19 3512-3169.
Figure S1. SDS-PAGE analysis of expression and purification of the recombinant protein
Bl1609Gal. (MW) molecular weight markers; (1) no IPTG induction; (2) after 4h of IPTG
induction; (3) Cellular extract after lysis; (4) sample obtained by nickel exchange
chromatography (5) sample obtained by size exclusion chromatography; (6) purified
BL1609Gal obtained after anion exchange chromatography.
Figure S2. GalB1 activity as a function of increased potato galactan concentrations. The
values of the kinetic constants were determined experimentally from the initial rates of
potato galactan hydrolysis at each substrate concentration under optimal activity
conditions.