Supplementary Information
Propaganda
Supplementary Information A new marseillevirus isolated in Southern Brazil from Limnoperna fortunei Raíssa Nunes dos Santos¹, Fabrício Souza Campos¹, Nathalia Rammé Medeiros de Albuquerque¹, Fernando Finoketti¹, Rayra Almeida Côrrea¹, Lucia Cano Ortiz¹, Felipe Lopes Assis², Thalita Souza Arantes², Paulo Michel Roehe¹, Ana Cláudia Franco¹. ¹ Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS). Avenida Sarmento Leite 500, SALA 315, Porto Alegre, CEP 90050-170, Rio Grande do Sul, Brasil. ² Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas da UFMG, Avenida Presidente Antônio Carlos, 6627 Caixa Postal 486, Belo Horizonte, CEP 31270-901, Minas Gerais, Brasil. *Corresponding author: Santos, R.N., Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS). Avenida Sarmento Leite 500, Porto Alegre, CEP 90050-170, Rio Grande do Sul, Brasil. +555133083655 E-mail [email protected] Phylogenetic reconstruction based on a alignment of the five core genes individually: DNA polymerase B, major capsid protein, VV-A18 helicase, D6/D11 helicase and D5 helicase. The amino acid sequences were aligned using Muscle. Evolutionary history was inferred by using the Maximum Likelihood method based on the JTT matrix-based model. FIGURE A) DNA polymerase B 100 74 Tunisvirus Lineage C Insectomine Brazilian marseillevirus 78 Lausannevirus 54 99 Lineage D Lineage B Port-miou Marseillevirus Tokyovirus Melbourne 97 96 Marseillevirus 100 Lineage A Senagalvirus 76 Cannes8 Golden marseillevirus Pandoravirus Faustovirus 53 73 Pithovirus Mollivirus Moumouvirus 97 77 90 Lineage B Mimivirus Lineage A Megavirus Lineage C Mimiviridae Figure B) VV-A18 helicase 100 90 95 62 100 98 99 100 58 70 94 100 62 99 73 Lausannevirus Lineage B Port-miou Lineage D Brazilian marseillevirus Insectomine Lineage C Tunisvirus Golden marseillevirus Tokyovirus Senagalvirus Marseillevirus Lineage A Cannes8 Melbournevirus Pithovirus Pandoravirus Mollivirus Faustovirus Lineage A Mimivirus Lineage C Mimiviridae Megavirus Lineage B Moumouvirus Maeseilleviridae Figure C) Major capsid protein Marseillevirus 99 Melbournevirus Lineage A Cannes8 76 Senagalvirus Tokyovirus Brazilian marseillevirus Lineage D 69 98 Lausannevirus Lineage B Port-miou 98 Insectomine Lineage C 99 Tunisvirus Golden marseillevirus 53 Pithovirus Mimivirus Lineage A Megavirus 87 93 Moumouvirus Mollivirus Faustovirus Lineage C Lineage B Mimiviridae Marseilleviridae Figure D) D5-HELICASE Insectomine Lineage C Tunisviruss Brazilian marseillevirus Lausannevirus Lineage D Lineage B Port-miou Marseilleviridae Golden marseillevirus Tokyovirus Cannes8 Senagalvirus Lineage A Marseillevirus Melbournevirus Pithovirus Mimivirus Megavirus Moumouvirus Faustovirus Lineage A Lineage C Lineage B Mimiviridae Figure E) D6/D11 HELICASE Senagalvirus Marseillevirus Lineage A Cannes8 Melbournevirus Tokyovirus Lausannevirus Marseilleviridae Lineage B Port-miou Brazilian marseillevirus Insectomine Lineage D Lineage C Tunisvirus Golden marseillevirus Mimivirus Megavirus Moumouvirus Pithovirus Faustovirus Lineage A Lineage C Lineage B Mimiviridae