Contacts

National Institute of Science and Technology – Genetic and
Health Information of Brazilian Animal Production
Area of research: Research on production, genetic and health potential of Brazilian Animal
Production
Home Institution:
Federal University of Minas Gerais - UFMG
Veterinary School
Structure of work groups
Institute Organizing Committee
Coordenator: Rômulo Cerqueira Leite – Professor/researcher - UFMG
Vice-coordenator: Concepta Margaret McManus - Professor/researcher - UNB
Executive Secretary: Janaína de Paula Coelho
Adjunct Secretary: Marc Henry - Professor/researcher - UFMG
Adjunct Secretary: Denise Aparecida Andrade de Oliveira - Professor/researcher - UFMG
Area Coordinators
Health and residues: Romário Cerqueira Leite - UFMG
Geomapping: João Paulo Haddad - UFMG
Genetics: Denise Aparecida Andrade de Oliveira - UFMG
Continuing education: Helder Louvandini - UNB
Public policies: Hélio Vilela Barbosa Júnior - MAPA
Sub-coordinators of areas:
Parasites: Romário Cerqueira Leite - UFMG
Aquatic organisms: Ana Cláudia Pinto Cottorelllo – LANAGRO - MG
Illnesses : Cristiano Barros de Melo - UNB
Health and residues in poultry : Nelson Rodrigues da Silva Martins - UFMG
Molecular genetics: Denise Aparecida Andrade de Oliveira - UFMG
Genetic moddelling : Concepta Margareth McManus Pimentel - UNB
Quantitative genetcs : Martinho de Almeida e Silva - UFMG
Reproduction genetics: Vera Fernanda Martins Hossepian de Lima – UNESP - Jaboticabal
Geomapping Land animals : João Paulo Haddad – UFMG
Geomapping aquatic animals : Daniel Crepaldi – IBAMA
Finance Committee:
Coordination: Marc Henry - UFMG;
Members: Denise Aparecida Andrade de Oliveira – UFMG;
João Paulo Haddad – UFMG;
Romário Cerqueira Leite – UFMG.
Scientific Committee
Coordination: Concepta Margaret McManus Pimentel – UNB;
Members: Andrea Micke Moreno- USP;
Otávio Mitio Ohashi – UFPA;
Nelson Rodrigo da Silva Martins – UFMG;
Josiane Tavares de Abreu – PUC - MG
Main research topics
The institute´s main objective is create and maintain an up-to-date data bank of information
which characterizes Brazilian animal production. The aim is to create a network of laboratories
in national territory which together develop, innovate and execute methods and means to
maintain and make available information which characterize diverse systems of production of
animals of economic interest. As such, the INCT is working in the following areas:
Characterization of geographic distribution of different production systems, genetic
characterization of species and breeds, studies on prevalence and spatial distribution of
infectious and parasite diseases. Molecular genotyping for characterization of reference strains
will be carried out as well as processes for genetic certification of products of animal origin,
selection assisted by markers and animal genetic forensics.
Summary of results obtained
The network of laboratories which support the INCT is being expanded. The studies began with
the geographical characterization of animal production systems. The municipal index of beef
cattle production development and aquatic organisms is in final phase of processing. Data on
swine and dairy cattle production are being developed. Thereafter the indices for poultry,
sheep, goats, buffaloes and horses as well as other systems will be developed. Together with
these indices, another front is collecting and cataloging all available information on the
incidence and prevalence of infectious diseases in Brazil as well as genetic characterization of
species of interest. The genetic characterization of some breeds of sheep, goats and horses has
been carried out where along with genetic evaluations, heat and parasite tolerance was
characterized. The results of geomapping of production systems will lead the way for new
studies. The genetic identification (barcode) of fish in the São Francisco river basin, is also
underway with 107 species already identified and another 143 in analysis. Genetic tests were
developed to detect fraud in milk products of buffalo origin. The INCT also developed
techniques for genetic identification of several fish species in natura, filleted and in posts for
fraud detection. The continuous actualization of the information bank is the challenge for the
INCT Genetic and Health Information of Brazilian Animal Production.
Production from May 2009 to August 2010
Genetics
Health
Geo
processing
Total
Publications
Articles published in national journals
24
23
25
21
Articles published in international journals
Studies presented in national congresses
Studies presented in international congresses
Formation of Human resources
Finished
Scientific Initiation
Masters
Doctors
Post-doctor
Others
1
3
1
1
4
11
5
3
1
4
15
9
1
1
On going
Scientific Initiation
Masters
Doctors
6
4
7
Post-doctor
9
9
6
3
3
1
1
18
16
14
1
Others
INCT Scholarships
Scientific Initiation
Technical support
Junior Postdoctorate
DTI 2
DTI 3
8
10
6
1
2
New members
Undergraduate
Masters
Doctors
Postdoctorate
Researcher
Institution
7
12
7
2
21
1
Ongoing research:
Genetics:
- Genetic dentification (Barcode) of fish from the São Francisco River Basin
- Development, Standardization and Implantation of DNA tests for genetic
Identification and Confirmation of Genealogies in Buffaloes.
- Genetic analysis of strains of Haemonchus contortus in cattle for identification of
resistence to benzimidazol.
- Development de genetic tests for fraud identification in meat and milk products.
- Development of microssatelites for Pirá (Conorhynchus conirostris) fish using
pirosequencing technqiue
- Technological innovations in the production of tilapia fry: triploids interploids.
- Development and use of de animal Genetic resources in Brazil using techniques in the
areas of reproduction, breeding, molecular biology, ethology and evaluation of products
and subproducts.
- Landscape genetics of sheep in Brazil: a georeferenced evaluation of genetic patterns
for conservation, characterization and rastreability of herds
- Integration of Population viability analyses (PVA) and molecular markers fro
conservation of animal genetic resources
- Validation of Scrapie susceptibility, prospection of new alleles and evaluation of
Brazilian sheep herd
- Implications for crosses in hair sheep in production, reproduction and health in
females
Health:
- Isolation and phylogenetic analysis of Brazilian samples of caprine arthritic
encephalitis (CAEV).
- Detection of infection by swine flu virus in swine in Minas Gerais.
- Diagnosis, Epidemiology and Control of illnesses in Poultry.
- Rational use of antiparasite medicine and prevention of residues in products of animal
origin: evaluation of alternative strategies and conventional methods of parasite control
in dairy cattle.
- Diagnóstico da resistência interespecífica em Haematobia irritans irritans (Linnaeus,
1758) (Diptera: Muscidae) and Rhipicephalus (Boophilus) microplus (Canestrini, 1887)
(Acari: Ixodidae) em decorrência da utilization de ectoparasiticidas em Minas Gerais.
- study of Parasitic otitis in Gyr herd in Minas Gerais.
Analysis and detection of etiological agents of transnational diseases in products of
animal origin in accompanied luggage on international flights in Tom Jobim (Rio de
Janeiro) and Franco Montoro (Guarulhos) airports
- Study of rabies cases in the Distrito Federal and surroundings in the last 10 years with
emphasis on human and cattle rabies
- Study of prevalence and risk factors for reproductive diseases in conservation herds of
Criuolo Lageano cattle in Brazil.
- Infection by Neospora caninum, Toxoplasma gondii and bovine leucose vírus in the
Catarinense Crioulo Lageno.
- Study of genetic variability of S1 gene of infectious bronchitis in poultry in Minas
Gerais
- Mapping of antigenic determinants of Brazilian isolates of infectious bronchitis in
chickens.
- Genotyping using RT-PCR/ RFLP for rapid classification of infectious bronchitis in
chickens from clinical outbreak in Brazil between 1972 and 2009, based on S1segment
- molecular characterization of Brazilian isolates of infectious bronchitis in chickens
- characterization of Brazilian isolates of infectious bronchitis in chickens based of
coding segments of S2 and N”
- Characterization of isolates of Mycobacterium bovis by conventional and RT PCR
- Sequencing of six samples of Aujeszky disease vírus (SuHV-1) from outbreaks in
Santa Catarina in 2002 and 2003
- Standardization of genotying methods for Aujeszky disease vírus (SuHV-1) using
PCR and restriction by BamHI;
- Standardization of PCR technique for detection of contamination of cell culture by
torque teno vírus (TTV);
- Standardization of PCR technique for detection of contamination of cell culture by
swine parvovírus (PPV);
- Detection of viral encephalitis by herpesvírus in 48 samples of bovine brain using
PCR multiplex.
- Standardization of PCR technique for detection of S. aureus and enterotoxins in Milk
and Milk products.
- Implantation of diagnosis using molecular biology in three marine shrimp viroses,
Mancha Branca, Infectious Mionecrose and IHHNV, allowing routine analysis.
- Evaluation of loading, transport and unloading cattle for well being
- Development and validation of analytical methods for determination and toxicological
evaluation of inorganic and chemical substances of vegetal and animal origin
- Evaluation of Mitigation of Methane and production in sheep at pasture fed with
tanniferous legumes
Geoprocessing
Current status
Beef cattle – finished - in publication
Milk cattle – in analysis
Swine – in analysis
Aquatic Organisms – concluded - in publication
Ovines – in analysis
- Methods of epidemological investigation: case Study of neosporosis and leptospirosis
in dairy cattle in the microregion of Sete Lagoas
- Development and validation of a system of simulation of infectious diseases in carrle
based on transport
- Movement of cattle and definition of collection areas of abattoirs under Federal
Insspection service (SIF) in the States of Mato Grosso, Mato Grosso do Sul, Paraná and
Santa Catarina
Sample bank
Genetic material
~ 5000 cattle,
~7800 horses,
~90 dogs,
~400 fish
~200 buffaloes
Microrganisms characterized
188 strains of Leptospira
33 strains of Clostridium
~400 agents that cause illnesses in aquatic organisms
75 + 55 strains of Brucellas
50 strains of virus
35 different cell lines
Prospection of reports of occurrence of animal diseases of
interest in Brazil
Status: advanced
Name
Bacterial diseases
Anthrax
Enzootic pneumonia
Black-leg
Botulism
Enterotoxaemia
Tetanus
Bacillary hemoglobinuria
Infectious necrotic hepatitis
Malignant edema
Bovine genital campylobacteriosis
Causative agent
Bacillus anthracis
Mycoplasma hyopneumoniae
Clostridium (feseri) chauvaei
Clostridium botulinum
Clostridium perfringens,
Clostridium difficile (Guinea
pigs)
Clostridium tetani
Clostridium haemolyticum (novyi
type D)
Clostridium novyi type B
Clostridium septicum
Campylobacter fetus venerealis
Bovine Brucellosis (Brucella abortus)
Caprine and ovine brucellosis
Porcine brucellosis (Brucella suis)
Brucellosis (Brucella suis biovar 4)
Ovine epididymitis (Brucella ovis)
Glanders
Leptospirosis
Listeriosis
Tuberculosis
Q-fever
Rickettsial diseases
Anaplasmosis
Bovine petechial fever
Ehrlichiosis
Heartwater
Equine granulocytic ehrlichiosis
Viral diseasses
Blue tongue
Bovine viral diarrhoea virus (BVD)
Buffalo pox
Classic Swine Fever
Enzootic bovine leukosis
Equine encephalomyelitis (Eastern)
Equine encephalomyelitis (Western)
Equine infectious anaemia
Foot-and-mouth disease
Infectious bovine rhinotracheitis/infectious
pustular vulvovaginitis
or C fetus fetus
Brucella abortus
Brucella melitensis
Brucella suis
Brucella suis biovar 4
Brucella ovis
Burkholderia mallei
Leptospira spp.
Listeria monocytogenes
Mycobacterium bovis
Coxiella burnetii
Anaplasma marginale
Ehrlichia ondiri
Ehrlichia phagocytophila
Ehrlichia (formerly Cowdria)
ruminantium
Anaplasma phagocytophila
Orbivirus
Pestivirus
Pox vírus
Flavivirus
Deltaretrovirus
Alphavirus
Alphavirus
Retrovirus (lentivirus)
Aphthovirus, family
Picornaviridae
Bovine herpesvirus 1
IHHNV, Parvovirus
Infectious hypodermal and hematopoietic
necrosis
Infectious Mionecrosis
Lumpy skin disease
Papillomatosis
Rabies
Vesicular stomatitis
West Nile fever
White Spot
Internal Parasites (helminths)
Cysticercosis (beef tapeworm)
IMNV, Totivirus
Pox vírus
Papillomavirus
Lyssavirus
vesiculoviroses - family
rhabdovirus
Arbovirus
WSSV, Whispovirus
Taenia saginata (Cysticercus
Cysticercosis (pork tapeworm)
Internal Parasites (protozoa)
Babesiosis
Coccidiosis
Cryptosporidiosis
Equine protozoal myeloencephalitis
Sarcocistosis
Sarcoide (Equine)
Toxoplasmosis
Trichomoniasis (cattle)
Prion diseases
Bovine spongiform encephalopathy
Chronic wasting disease
Scrapie
bovis)
Taenia solium (Cysticercus
cellulosae)
Babesia bigemina, B. bovis
Eimeria spp.
Cryptosporidium species
Sarcocystis neurona
Sarcocystis cruzi
Papillomavirus
Toxoplasma gondii
Tritrichomonas foetus foetus
Prion
Prion
Prion
Courses offered by INCT
Atualization of genetic Identification tests and Confirmation of Genealogies.
Introduction to SAS
Methods of Experimentation in Animal Science
Courses taken by members of INCT
- Analysis of fragments by capillar electrophorisis
- CBAB in molecular biology techniques applied to diagnosis of parasite resistence in
helminths of veterinary importance.
- sequencing by capillar electrophorisis.
- Quantitativ Real Time PCR
- Analysis of fragments and sequencing by capillar electrophorisis on an ABI 3130.
INCT Key note speeches – n=3
Contacts
Telephone: 55 31 3409-2073
E-mail: [email protected]
Address: Avenida Antônio Carlos, 6627, Pampulha - Belo Horizonte - Minas Gerais
CEP: 30123-970 / Caixa Postal 567
Site: www.animal.unb.br