BioECO – CESAM Maio de 2010

CESAM – Lisboa
Environmental Biology and Health
Subgroup
Maria Filomena Caeiro
Fevereiro de 2013
Nucleo-cytoplasmic large DNA viruses (NCLDVs) and their
pathogenic potencial
In collaboration with António Pedro Alves de Matos (CESAM) & Rachel
Marschang (Hohenheim University, Stuttgart, Germany)
Characterization of ranaviruses isolated from the Portugese fauna
Ana Mafalda Dinis
Master thesis on Applied Microbiology (concluded)
Nucleo-cytoplasmic large DNA viruses: detection and characterization of
viruses in environmental amoeba
Mariana Costa Fugas
Master thesis on Molecular Biology and Genetics (concluded)
Mara Maeva Meirsman & Vera Mónica Brito Salgado
IIM2 – 2nd semester Master on Applied Microbiology (underway)
Ana Mendes & Maria Silva
Undergraduate students
Alves De Matos A.P., Caeiro M.F., Papp T., Matos B.A., Correia A.C. & R.E. Marschang (2011). New viruses from Lacerta
monticola (Serra da Estrela, Portugal): further evidence for a new group of nucleo-cytoplasmic large deoxyriboviruses
(NCLDVs). Microscopy and Microanalysis 17: 101–108 and CJO 08 Dec 2010 (Doi:10.1017/S143192761009433X).
Cinéticas dos ciclos replicativos dos isolados de ranavírus portugueses
e do FV3
1E+10
Ma3A
100000000
Ma3A
extra
10000
100
Ma3A
intra
1
2 4 6 8 10121416182448
Hora da colheita (pi)
Vírus Extracelular
Vírus Intracelular
10000
Ma3A
Ma3B
Ma3P
BoA
100
1
2 4 6 8 10 12 14 16 18 24 48
Hora da colheita(pi)
1E+10
100000000
1000000
10000
100
1
Título viral (pfu/ml)
1000000
Título viral (pfu/ml)
Título viral (pfu/ml)
1000000
Ma3A
Ma3B
Ma3P
BoA
LMO
FV3
2 4 6 8 10121416182448
Hora da colheita(pi)
A
B
C
D
E
F
G
A
B
C
D
E
F
G
H
H
Figure 1 – Free living amoeba isolated from AW1, AW2, Granjal,
Ferragudo, LO1 and LO2 collected samples. All images were
obtained in bright field. The amoebas were termed after the sample
from where they were isolated: A - AW1 trophozoite; B – AW2
trophozoite; C – GH trophozoite; D – GP trophozoite; E – Fer
trophozoite; F – LO1 trophozoite; G – LO2 cyst; H – LO2 trophozoite.
Figure 2 – Free living amoeba isolated from the Tagus River region.
All images were obtained in bright field. The amoebas were named by
numbers corresponding to a specific sample and media in which they
grew (see Table 2): A – 1 trophozoite; B – 2 cyst; C – 2 trophozoite; D –
3 cyst; E – 3 trophozoite; F – 4 trophozoite and cyst; G – 5 trophozoite; H
– 6 cyst.
Detection, isolation and characterization of viruses
involved in human and animal health
Detection and characterization of viruses circulating in Portuguese
populations of Alectoris rufa and other game birds.
In collaboration with Deodália Dias (CESAM)
Maryline Dias
Master thesis on Human Biology and Environment (concluded)
• Paper under submission:
• Coronaviruses detection in healthy Alectoris rufa in Portuguese breeding properties. Dias M., Dias D. & Caeiro M.F.
Looking for viruses in animal tissues from natural populations of
rodents
In collaboration with Cristiane Bastos-Silveira (CESAM)
Alexandra Carvalho
Master thesis on Applied Microbiology (concluded)
• Attempts on the isolation of the Portuguese EMCV in cell cultures
• Paper under submission:
• First molecular detection of Encephalomyocarditis virus in a Lusitanian pine vole (Microtus lusitanicus) in
Portugal. Carvalho A., Cerveira A., Bastos-Silveira C. & Caeiro M.F.
Studies on antiviral activities of aqueous plant extracts
In collaboration with Lia Ascenção (CBV) & Luísa Serralheiro (CQB) &
António Pedro Alves de Matos (CESAM)
• Virucidal effect against:
Herpes simplex viruses (Type 1 and type 2)
Encephalomyocarditis virus (EMCV)
Ranaviruses (FV3 and Portuguese isolates from
diseased animals: Triturus marmuratus and
Triturus boscai)
• Effect on the replication cycles
Fernanda Resende
Master thesis on Applied Microbiology (concluded)
Nadieny Barbosa
Master thesis on Molecular Biology and Genetics (undeway)
Mónica Barra
Master thesis on Applied Microbiology (underway)
Resultados
Citotoxicidade
Resultados
Estudo da atividade anti-herpética – Efeito virucida
Resultados
Efeito no ciclo replicativo (tempos de adição)
Representação gráfica (Microsoft Excel) da influência do tempo de adição dos extratos (CMNC),
durante a adsorção e a diferentes tempos pós infeção, na percentagem de inibição da produção de
partículas virais infecciosas (HSV-1, HSV-2).
Molecular methods applied to fungal identification
and detection
Diversity of marine fungi on the Portuguese west coast
In collaboration with Margarida Barata (CBA)
Egídia Azevedo
PhD thesis (concluded)
- Azevedo E., Rebelo R., Caeiro M. F. & Barata M. (2010). Diversity and Richness of marine fungi on two
Portuguese marinas. Nova Hedwigia. 90 (3-4): 521- 531
- Azevedo A., Caeiro M.F., Rebelo R. & Barata M. (2011) Biodiversity and characterization of marine mycota from
Portuguese waters. Animal Biodiversity and Conservation 34.1: 205-215
- Azevedo E., Rebelo R., Caeiro M.F. & Barata M. (2012). Use of drift substrates to characterize marine fungal
communities from the west coast of Portugal. Mycologia 2012 11-191; Published online: January 12, 2012,
doi:10.3852/11-191 (in press)
- Azevedo E., Barata M., Marques M.I.& Caeiro M.F. Lignicolous Lulworthia spp. isolated in Portuguese waters:
characterization and phylogenetic analysis (under submission)
Rhizospheric microbial consortia to increase nutrient use efficiency. A tool
to be used in intensive farm systems (PTDC/AGR-PRO/115888/2009)
In collaboration with Cristina Cruz (CBA) - PI of the Project
Manuscript in preparation:
•
Next generation sequencing for metagenomics of endophytic fungi. Delgado M., Antunes P., Cruz C.
& Caeiro M.F.
PHYLOGENETIC TREE
FOR PORTUGUESE
LULWORTHIALES
Sequences:
•Aligned with Clustalw
•Phylip analysis:
•Dnadist
•Njtree
•1000 bootstrap
Azevedo E., Barata M.
Marques M.I. & Caeiro M.F.
(under submission)
Endophytic fungi of maize:
Metagenomic analysis
Delgado M., Antunes P.,
Cruz C. & Caeiro M.F.
(2013) in preparation
Endophytic fungi of maize:
Metagenomic analysis
Delgado M., Antunes P.,
Cruz C. & Caeiro M.F.
(2013) in preparation